Organic Residue Analysis in Cosa, Italy
Creators
- 1. CRC 1070 ResourceCultures
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2.
University of Freiburg
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3.
University of Bonn
Description
The dataset contains the data associated with organic residue analysis (ORA), including chromatograms in .csv and .MzML formats, alongside an Excel table detailing sampling inormation. This repository entry pertains to the ORA analysis, conducted in the laboratory of Tübingen under the supervision of Prof. Dr. Maxime Rageot (currently at the University of Bonn).
The primary objective of this ORA analysis was to support the hypothesis that the analysed Cocciopesto was part of the floor of a wine press.
The protocol used for the solvent lipid extraction is the one explained at Mottram et al. 1999; and the protocol used for the acid extraction is the one from Garnier & Valamoti 2016.
Technical info
Analysis
The analysis was performed by Gas Chromatography (GC) and GC-Mass Spectrometry (GC-MS) using an Agilent Technologies 7890B GC System series chromatograph including Agilent Technologies Capillary Flow-Technology Three-Way Splitter Kit coupled to an Agilent Technologies 5977A MSD and FID. The analyses were carried out using helium as a carrier gas, with a split/splitless injection system (Gerstel Multi-Purpose-Sampler and Gerstel Cold-Injection-System 4), operating in the splitless mode with a purge flow of 3.0ml min–1 and a constant pressure at the head of the column of 8.6667 psi. Samples were analysed using an Agilent J&W DB-5HT-column (15m × 0.32mm i.d.; 0.1μm film thickness) and divided in two equal parts using 0.18mm non-coated, deactivated silica capillary columns (0.66m splitter-column to FID/ 1.52m splitter-column to MSD) with the Three-Way Splitter Kit. The inlet temperature was ramped from 30°C to 240°C at 12°C s-1 (held isothermally for 5min) and then increased to 350°C at 12°C s-1 (held isothermally for 10min). The temperature of the oven was set at 50°C for 1min followed by an increase to 100°C at 15°C min–1, then to 240°C at 6°C min–1 and to 350°C at 10°C min–1 (held isothermally for 20min). Mass spectra were acquired using electron ionization at 70 eV and obtained by scanning between m/z 50–950 in 1.562s. The interface and the ion source temperatures were 300°C and 280°C, respectively. The temperature of the FID detector was fixed at 340°C. Mass spectra were matched against the National Institute of Standards and Technology (NIST) library, 2014 edition.
Files
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Additional details
Funding
Data quality
- Accuracy
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The samples were measured with an Agilent Technologies 7890B GC System series chromatograph including a three-way splitter kit with Agilent Technologies capillary flow technology coupled to an Agilent Technologies 5977A MSF and FID.
- Completeness
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Not applicable.
- Conformity
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Not applicable.
- Consistency
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Not applicable.
- Credibility
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Not applicable.
- Processability
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Not applicable.
- Relevance
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This dataset is relevant to the fields of archaeology and biomolecular archaeology, especially for the Roman research in Italy related to economy and agriculture.
- Timeliness
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Not applicable.
- Understandability
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This dataset is understandable for archaeologists and chemists working in the fields of archeology and biomolecular archaeology.